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. 2009 Sep 15;101(8):1433–1443. doi: 10.1038/sj.bjc.6605316

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Copyright © 2009 Cancer Research UK

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Enforced expression of FOXG1 blocks the induction of p21^WAF1/CIP1 and promotes cell proliferation on TGF-β treatment. (A, B) Western blot analysis showed the expression of Flag-tagged FOXG1 in two stable clones, SK-C18 and Acp-C4, of SKOV3 and A2780cp ovarian cancer cell lines, respectively. SK-V and Acp-V are their corresponding vector controls. (C) The vector controls and Flag-tagged FOXG1 stable clones of SKOV3 (left) and A2780cp (right) were cultured in the presence (+) or absence (−) of 100 pM TGF-β for 24 h. The p21^WAF1/CIP1 mRNA and protein levels were analysed by semi-quantitative RT–PCR and western blot analyses, respectively. (D) The vector controls and Flag-tagged FOXG1 stable clones of SKOV3 (left) and A2780cp (right) were cultured in media containing 10 pM TGF-β for 5 days. XTT assay showed lower cell proliferation rate in FOXG1-overexpressed clones (38% for SK-C18, P<0.05; and 48% for Acp-C4, P<0.01) as compared with their vector controls.