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. 2009 Nov 4;4(11):e7756. doi: 10.1371/journal.pone.0007756

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Mücke et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The contour lengths of the simulated chains was set to 4 x L_P and of the IFs 3 x to 7 x L_P, respectively. Brownian dynamic simulations were performed for weak (a) and strong (b) filament surface interaction. If weak filament surface interaction was taken into account smooth contours with random bending can be observed (a). If strong filament surface interactions were taken into account (b) loops can be found and the curvature does not appear random. Traced vimentin IF contours deposited on modified glass (c) and freshly cleaved mica (d) are shown. After image processing (see Material and Methods), representative contours have been selected and arranged in an image. Image size: 10 x L_P.