Table 2. Polymer statistics of the simulated deposition scenarios in comparison to the normalized apparent persistence length of obtained filaments adsorbed to different supports.
surface | <s>LP a | LP<cos(Θ)>/LP | LP<Θ2>/LP | LP<R2>/LP | correlation |
simulated | filamentsb | LP = 1 | |||
strong | 1 | 0.44 | 0.37 | 0.44 | Yes/0.02 |
strong | 2 | 0.40 | 0.29 | 0.39 | Yes/0.04 |
strong | 4 | 0.32 | 0.20 | 0.32 | Yes/0.10 |
Vimentin | LP = 1050 nm | ||||
Mica | 1.0 | 0.32 | 0.33 | 0.42 | Yes/0.02 |
Mica | 2.5 | 0.30 | 0.28 | 0.35 | Yes/0.07 |
Mica | 3.9 | 0.31 | 0.17 | 0.30 | Yes/0.12 |
All mean errors were less than 1% in case of simulated filaments and less than 5% for the IFs. No correlation was found for the equilibrated vimentin filaments. The integral is 0.00 for both lengths.
Length s of the filaments divided by the persistence length LP (assumption: full equilibrated); LP is the mean value of all persistence lengths measured with weak filament-protein interaction.
2000 counts.