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Comparison of Trp4 fluorescence intensity of Cu(II)-F4W and Cu(II)-F4W/H50S α-syn ([α-syn] = [Cu(II)] = 1 μM) as a function of pH in deoxygenated 20 mM MOPS, 100 mM NaCl buffer. Error bars represent standard deviations. Fits are shown as solid lines. (Inset) Representative Trp fluorescence data (buffer background has been subtracted).
