Table 3.
Myelin markers found to be significantly regulated in CPZ treated rats. Fold-change was calculated with RMA. P-value was calculated with log2 transformed data. Affymetrix probe set IDs and Entrez gene ID numbers are provided. Transcripts shown in Figure 3 are denoted with ‘x’.
| Myelin markers (abbreviations) | fold change | P value (log2) | P call % of control | P call % of cuprizone | Entrez Gene ID | Affymetrix probe set ID | Verified with Q-PCR |
|---|---|---|---|---|---|---|---|
| myelin-associated glycoprotein (Mag) | −1.8 | 0.0002 | 100 | 100 | 29409 | 1368861_a_at | x |
| myelin-associated oligodendrocytic basic protein (Mobp) | −2.1 | 0.0000 | 100 | 100 | 25037 | 1368263_a_at | x |
| myelin-associated oligodendrocytic basic protein (Mobp) | −3.5 | 0.0025 | 100 | 100 | 25037 | 1370500_a_at | |
| myelin-associated oligodendrocytic basic protein (Mobp) | −3.4 | 0.0000 | 100 | 100 | 25037 | 1370434_a_at | |
| myelin basic protein (Mbp) | −1.8 | 0.0254 | 100 | 100 | 24547 | 1387341_a_at | x |
| myelin basic protein (Mbp) | −1.4 | 0.0002 | 100 | 100 | 24547 | 1368810_a_at | |
| myelin oligodendrocyte glycoprotein (Mog) | −2.0 | 0.0002 | 100 | 100 | 24558 | 1398257_at | x |
| myelin oligodendrocyte glycoprotein (Mog) | −1.3 | 0.0114 | 100 | 60 | 24558 | 1377352_at | |
| myelin and lymphocyte protein (Mal) | −3.8 | 0.0000 | 100 | 100 | 25263 | 1387040_at | |
| oligodendrocyte transcription factor 1 (Olig1) | −1.3 | 0.0005 | 100 | 100 | 60394 | 1387200_at | |
| plasma membrane proteolipid (Pllp) | −1.3 | 0.0138 | 100 | 100 | 64364 | 1386943_at | |
| proteolipid protein (Plp) | −1.6 | 0.0006 | 100 | 100 | 24943 | 1387112_at | x |
| claudin 11 (Cldn11) | −1.9 | 0.0305 | 100 | 100 | 84588 | 1369609_at | |
| claudin 11 (Cldn11) | −1.5 | 0.0029 | 100 | 100 | 84588 | 1376711_at | |
| CD9 antigen (Cd9) | −1.3 | 0.0119 | 100 | 100 | 24936 | 1371499_at | |
| v-erb-b2 erythroblastic leukemia viral oncogene homolog (Erbb3) | −1.6 | 0.0037 | 100 | 100 | 13867 | 1377821_at | |
| gelsolin (Gsn) | −1.4 | 0.0007 | 100 | 100 | 296654 | 1371414_at | |
| kallikrein 6 (Klk6) | −2.8 | 0.0000 | 100 | 40 | 29245 | 1368384_at | |
| response gene to complement 32 (Rgc32) | −1.3 | 0.0001 | 100 | 100 | 117183 | 1368080_at | |
| developmentally regulated protein TPO1 | −1.2 | 0.0479 | 100 | 100 | 170907 | 1386979_at | |
| transferrin | −1.6 | 0.0027 | 100 | 100 | 24825 | 1370228_at | |
| tetraspanin 2 (Tspan2) | −1.5 | 0.0002 | 100 | 100 | 64521 | 1368104_at | |
| tetraspanin 2 (Tspan2) | −1.4 | 0.0008 | 100 | 100 | 64521 | 1368105_at | |
| UDP galactosyltransferase 8 (Ugt8) | −2.3 | 0.0001 | 100 | 100 | 50555 | 1368858_at | |
| aspartoacylase (Aspa) | −1.4 | 0.0000 | 100 | 100 | 79251 | 1368563_at | |
| Astrocyte Marker | |||||||
| glial fibrillary acidic protein (Gfap) | 1.4 | 0.0128 | 100 | 100 | 24387 | 1368353_at | x |