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. Author manuscript; available in PMC: 2010 Sep 8.

Published in final edited form as: Circulation. 2009 Aug 24;120(10):888–896. doi: 10.1161/CIRCULATIONAHA.108.833491

Phosphorylated protein expression in brain cortex and left ventricle 15 min after sham operation or CA/CPR in mice treated with vehicle or Na₂S 1 min before CPR (Veh and Na₂S, respectively) or Na₂S administered at 10 min after CPR (post-CPR Na₂S). Relative phospho-Akt^Ser473 levels were quantitated by dividing the phospho-Akt ^Ser473 immunoreactivity by total Akt immunoreactivity and normalized to values of sham-operated mice in the heart (Panel A) and brain (Panel B). Relative phospho-NOS3 ^Ser1179 levels were quantitated by dividing the phospho-NOS3 ^Ser1179 immunoreactivity by total NOS3 immunoreactivity and normalized to values of sham-operated mice in the heart (Panel C) and brain (Panel D). Relative phospho-AMPKα ^Thr172 levels were quantitated by dividing the phospho- AMPKα ^Thr172 immunoreactivity by total AMPKα immunoreactivity and normalized to values of sham-operated mice in the brain (Panel E). Two representative blots are shown out of 6–8 blots in each group for all proteins. Loading controls are shown to demonstrate equal sample loading. *P<0.05 vs sham. #P<0.05 vs vehicle, †P<0.05 vs Na₂S. Panel F. Survival during the first 24h after cardiac arrest and CPR in NOS3^−/− mice treated with vehicle (NOS3^−/−+ Vehicle, n=7) and NOS3^−/− mice treated with Na₂S (NOS3^−/−+ Na₂S, n=7).

Phosphorylated protein expression in brain cortex and left ventricle 15 min after sham operation or CA/CPR in mice treated with vehicle or Na₂S 1 min before CPR (Veh and Na₂S, respectively) or Na₂S administered at 10 min after CPR (post-CPR Na₂S). Relative phospho-Akt^Ser473 levels were quantitated by dividing the phospho-Akt ^Ser473 immunoreactivity by total Akt immunoreactivity and normalized to values of sham-operated mice in the heart (Panel A) and brain (Panel B). Relative phospho-NOS3 ^Ser1179 levels were quantitated by dividing the phospho-NOS3 ^Ser1179 immunoreactivity by total NOS3 immunoreactivity and normalized to values of sham-operated mice in the heart (Panel C) and brain (Panel D). Relative phospho-AMPKα ^Thr172 levels were quantitated by dividing the phospho- AMPKα ^Thr172 immunoreactivity by total AMPKα immunoreactivity and normalized to values of sham-operated mice in the brain (Panel E). Two representative blots are shown out of 6–8 blots in each group for all proteins. Loading controls are shown to demonstrate equal sample loading. *P<0.05 vs sham. #P<0.05 vs vehicle, †P<0.05 vs Na₂S. Panel F. Survival during the first 24h after cardiac arrest and CPR in NOS3^−/− mice treated with vehicle (NOS3^−/−+ Vehicle, n=7) and NOS3^−/− mice treated with Na₂S (NOS3^−/−+ Na₂S, n=7).