Macrophage-Specific Expression of Human Lipoprotein Lipase Accelerates Atherosclerosis in Transgenic Apolipoprotein E Knockout Mice but Not in C57BL/6 Mice

. Author manuscript; available in PMC: 2009 Oct 27.

Published in final edited form as: Arterioscler Thromb Vasc Biol. 2001 Nov;21(11):1809–1815. doi: 10.1161/hq1101.097805

Lipase Activity in Culture Media of Peritoneal Macrophages

Mouse Line	Genotype	Lipase Activity, mmol/L FFA · min⁻¹ · mg⁻¹ (n±3)	P
3717/4	huLPL−	5.43±0.36	<0.03
	huLPL+	7.12±0.78
4676/3	huLPL−	3.12±0.56	<0.0001
	huLPL+	9.33±0.45
4697/3	huLPL−	7.49±0.08	<0.0001
	huLPL+	14.86±0.86
4699/1	huLPL−	7.19±0.87	<0.002
	huLPL+	24.34±4

FFA indicates free fatty acid.

Resident mouse macrophages were isolated from each mouse line. Cells from 3 to 7 mice with either huLPL+ or huLPL− genotype were pooled and cultured as in Figure 1. Lipase activity in culture media was determined as in Methods. Results are corrected for cell number by use of total cellular protein.