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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: Leuk Res. 2007 Jan 23;31(8):1145–1148. doi: 10.1016/j.leukres.2006.12.006

Figure 2. Agarose gel electrophoresis of RT-PCR products demonstrating MLL-SEPT9 fusion.

Figure 2

Lanes: 100 bp molecular weight ladder (New England Biolabs, Cambridge, Massachusetts); 1, MDS patient sample RT-PCR product, amplified using primer pair: 5′-CCTCTTGCTCCACCCATCAAAC-3′ (MLL exon 6, sense strand) and 5′-CGCCCAGGTCCTGGAATTT-3′ (SEPT9 exon 3, antisense); 2, wild-type control with same primer pairs; 3, patient PCR product using primer pair: MLL exon 6, sense, as above, and 5′-TTCTCCACCTGCTTGGACGAG-3′ (SEPT9 exon 3, antisense); 4, wild-type control with same primer pairs. Transcript variant 3 (Figure 3) is predicted to have a 608 bp product using the primer set in lane 3 and 448 bp product using the primer set in lane 1, which is what is observed. Product identity was verified by DNA sequencing.