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. Author manuscript; available in PMC: 2010 Mar 20.
Published in final edited form as: Cell. 2009 Mar 20;136(6):1056–1072. doi: 10.1016/j.cell.2008.12.040

Figure 6. Diminished FAS induction leading to blunted de novo lipogenesis and decreased triglyceride levels in liver and serum.

Figure 6

(A) Nascent RNA from liver nuclei were used for RT-PCR. (B) Nascent RNA from liver nuclei were used for RT-qPCR. Fold induction normalized by β-actin was shown. (C) Run-on of biotin labeled nascent transcripts from liver nuclei at different time points upon feeding were analyzed by RT-qPCR. (D) ChIP for biotin incorporation and indicated protein binding to the FAS promoter in liver. (E) Newly synthesized fatty acids during a 4 hr-period of body water labeling with 2H2O in livers from 9-wk old WT and SCID mice during feeding were measured. Values are means ± SEM, n = 12. (F) Equal amounts of Liver extracts from 9-wk old WT and SCID mice after 24 hrs of feeding were immunoblotted for total FAS protein levels. (G) Hepatic and serum triglyceride levels were measured in 9-wk old fed WT and SCID mice. (H) Schematic representation of USF-1 and its interacting partners and their effects on lipogenic gene transcription in fasting/feeding.