Table 5.
Analyses of variance for Tb and Hsp70 expression in field-collected beetles
| Source | Adult body temperature
|
Hsp70 expression
|
||||
|---|---|---|---|---|---|---|
| df | MS | F | df | MS | F | |
| Drainage (D) | 1 | 13.40 | 3.98* | 2 | 150,094 | 25.04‡ |
| Elevation (E) | 2 | 23.41 | 6.95† | 2 | 31,935 | 5.33† |
| Interval (I) | 4 | 17.49 | 5.19‡ | 4 | 26,318 | 4.39† |
| Gender (G) | 1 | 2.92 | 0.87 | 1 | 12,424 | 2.07 |
| D × E | 2 | 16.30 | 4.84* | 4 | 2,585 | 0.43 |
| D × I | 4 | 5.70 | 1.69 | 8 | 16,719 | 2.79† |
| D × G | 1 | 3.53 | 1.05 | 2 | 865 | 0.14 |
| E × I | 8 | 6.39 | 1.90 | 8 | 8,771 | 1.46 |
| E × G | 2 | 0.48 | 0.14 | 2 | 5,570 | 0.93 |
| I × G | 4 | 3.09 | 0.92 | 4 | 2,762 | 0.46 |
| D × E × I | 8 | 38.91 | 11.55‡ | 16 | 8,354 | 1.39 |
| E × I × G | 8 | 3.94 | 1.17 | 8 | 4,386 | 0.73 |
| D × E × G | 2 | 1.28 | 0.38 | 4 | 2,697 | 0.45 |
| D × I × G | 4 | 2.43 | 0.72 | 8 | 6,636 | 1.11 |
| D × E × I × G | 8 | 4.92 | 1.46 | 16 | 6,243 | 1.04 |
| Air temperature | 1 | 33.15 | 9.84† | — | — | — |
| Error | 60 | 3.37 | 90 | 5,995 | ||
Hsp70 levels are included for beetles from all three drainages whereas Tb from only BPC and BC beetles are included. *, P < 0.05; †, P < 0.01; ‡, P < 0.001; dg, degrees of freedom; MS, mean square; F, F ratio.