Table 1.
| Charge State Assignment Methods, Number of correct assignments | |||||
|---|---|---|---|---|---|
| Complex (Buffer) [Collision energy] | R2 | Absolute value of the slope | Conventional ESI | First positive slope | Replicates |
| SsDps-L (TEAA pH 6.8) [CE 50] | 1 | 2 | 0 | 3 | 3 |
| SsDps-L (AA pH 6.8) [CE 50] | 1 | 3 | 3 | 3 | 3 |
| SsDps-L (AA pH 8.2) [CE 50] | 0 | 3 | 3 | 3 | 3 |
| SsDps-L (AB pH 6.8i 7.4f) [CE 50]* | 1 | 0 | 3 | 3 | 3 |
| SsDps-L (AB pH 8.3) [CE 50]** | 3 | 0 | 3 | 3 | 3 |
| SsDps-L (TEAA pH 8.3i 7.7f) [CE 50]* | 0 | 1 | 0 | 2 | 2 |
| LiDps (AA pH6.8) [CE 50] | 1 | 3 | 3 | 3 | 3 |
| LiDps (AB pH8.2) [CE 50] | 0 | 3 | 3 | 3 | 3 |
| LiDps (AA pH8.7) [CE 50] | 0 | 3 | 3 | 3 | 3 |
| LiDps (TEAA pH8.3) [CE 50] | 1 | 3 | 2 | 3 | 3 |
| LiDps (TEAA pH6.8) [CE 50] | 2 | 2 | 2 | 2 | 2 |
| LiDps (TEAA pH 6.8) [CE 30] | 0 | 3 | 3 | 3 | 3 |
| LiDps (TEAA pH 6.8) [CE 50] | 1 | 3 | 3 | 3 | 3 |
| LiDps (TEAA pH 6.8) [CE 70] | 2 | 3 | 3 | 3 | 3 |
| LiDps (TEAA pH 6.8) [CE 90] | 2 | 2 | 2 | 2 | 2 |
| LiDps (TEAA pH 6.8) [CE 110] | 1 | 2 | 2 | 2 | 2 |
| LiDps (TEAA pH 6.8) [CE 130] | 0 | 1 | 2 | 2 | 2 |
| All Data | Correct for 35% | Correct for 80% | Correct for 87% | Correct for 100% | 46 |
*
“i” indicates the pH prior to injection of the first replicate and “f” indicates the pH after the final injection.
**
Data not used to produce the histogram in Figure 6d.