a, Western blot analysis using Bves and GAPDH primary antibodies shows increase in Bves expression after TB4 treatment in the non infarcted cardiac tissue 24 h after ligation. b, Densitometric analysis of Western blot results (a) normalized to GAPDH loading control. Bars indicate standard deviation at 95% confidence limits (n=6). c-f, Immunohistochemical analysis with anti-Bves antibody shows increase in Bves-positive cells and organ-wide thickening of the remote epicardium 3 days after systemic TB4 treatment (c) compared to PBS (e). (d,f) are DAPI stain of (c,e). ec, epicardium