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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: J Mol Cell Cardiol. 2009 May;46(5):728–738. doi: 10.1016/j.yjmcc.2009.01.017

Figure 6. p-Marcks labels future endothelial and smooth muscle cells in the adult epicardium.

Figure 6

a-c, Immunocytochemical analysis of p-Marcks positive epicardial cells with sm α-actin (b) and p-Cytokeratin (c) primary antibodies indicate endothelial and/or smooth muscle cell fate seven days after culturing. (a) endothelial marker positive cells highlighted by green arrowheads, smooth muscle marker positive cells highlighted by red arrowheads. d-i, sm α-actin, Cytokeratin, Pecam-1 (red) and p-Marcks (green) co-immunostaining with DAPI (blue) reveals cellular heterogeneity in the TB4 activated (d,f,h) or in the single layered epicardium (e,g,i) of adult control hearts. White arrowheads in d-i indicate p-Marcks positive cells with endothelial or smooth muscle cell fate in the developing capillaries. j-l, Immunohistochemical analysis of the mature coronary vessels suggest smooth muscle and endothelial cell fate for p-Marcks positive cells. ec, epicardium; e, endothel; sm, smooth muscle; m, mesenchyme