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. Author manuscript; available in PMC: 2010 Mar 1.

Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Mar;114(1-2):8–20. doi: 10.1016/j.jsbmb.2008.12.023

Fig 4 — Physical association of XBP1 and ERα is accompanied by robust ERE-driven transcriptional activity in MCF7/XBP1 cells. A, MCF-7 cells stably expressing XBP1 cDNA or the empty vector control (c) were treated with FAS or ethanol control (ctrl.) vehicle prior to lysis and immunoblotting (lanes 1 and 2) or co-immunoprecipitation of XBP1 and ERα (lanes 3 and 4) using standard procedures. B, MCF7/c and MCF7/XBP1 cells were transiently co-transfected with plasmids encoding 3xERE-luciferase and phRLSV40-Renilla for 24 hours prior to lysis and promoter-reporter luciferase assay by standard methods. Data are presented as mean relative ERE-luciferase activity ± SE for a representative experiment performed in triplicate, *p<0.001.

Fig 4 — Physical association of XBP1 and ERα is accompanied by robust ERE-driven transcriptional activity in MCF7/XBP1 cells. A, MCF-7 cells stably expressing XBP1 cDNA or the empty vector control (c) were treated with FAS or ethanol control (ctrl.) vehicle prior to lysis and immunoblotting (lanes 1 and 2) or co-immunoprecipitation of XBP1 and ERα (lanes 3 and 4) using standard procedures. B, MCF7/c and MCF7/XBP1 cells were transiently co-transfected with plasmids encoding 3xERE-luciferase and phRLSV40-Renilla for 24 hours prior to lysis and promoter-reporter luciferase assay by standard methods. Data are presented as mean relative ERE-luciferase activity ± SE for a representative experiment performed in triplicate, *p<0.001.