Fig. 4.

c-Abl and its PxxP motif interaction partners regulate the spreading speed of cells. NIH3T3 cells overexpressing Grb2, Nck2, CrkII, or c-Abl (A and B), Abl DKO cells re-expressing various forms of Abl (C and D), and Crk family knockdown NIH3T3 cells (G and H), were plated on fibronectin-coated surface and fixed at 10 minutes when cells were actively spreading. Nck family knockdown or STI-571-treated NIH3T3 cells were fixed at 9 minutes (E and F). A, C, E, and G. Spreading cells were stained for F-actin and DNA. Infected cells express EGFP (green) as marker (A). White bar = 50 μm. B, D, F, and H. Cell areas for the stained cells were measured. Each bar represents mean and S.E.M. of the cell area. ‡ indicates P<0.05.