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. Author manuscript; available in PMC: 2010 Sep 4.
Published in final edited form as: Cell. 2009 Sep 4;138(5):976–989. doi: 10.1016/j.cell.2009.06.051

Figure 1. Generation of Tph2−/− mice.

Figure 1

(A) β-Galactosidase staining in the mouse brain during embryonic (E12.5–18.5) development. A: Anterior; P: Posterior.

(B) Localization of Tph2-expressing neurons in the Dorsal (DR; from Bregma −4.04 to −5.49), Median (MR; from Bregma −4.04 to −4.48) and Caudal raphe (CR; from Bregma −4.84 to −7.48) in coronal sections of a mouse brain.

(C) Tph2 expression by in situ hybridization, β-galactosidase staining and co-immunolocalization in Tph2LacZ/+ mice. Arrowheads indicate Tph2/ β-Gal double positive cells.

(D) Real-time PCR (qPCR) analysis of Tph2 expression in tissues of WT mice.

(E) qPCR analysis of Tph2 expression in brainstem (BS) and duodenum (Duod) of WT and Tph2−/− mice.

(F) HPLC analysis of serotonin levels in different regions of brain in WT, Tph2+/− and Tph2−/− mice.

(G) Serum serotonin levels in WT, Tph2+/− and Tph2−/− mice.

(H) Mean litter size, serum biochemistry and body length in WT, Tph2+/− and Tph2−/− mice (n is indicated in superscript above each value).

All panels (except F) * P < 0.05; ** P < 0.01 (Student’s t test). Error bars, SEM. Panel F (One way ANOVA, Newman-Keuls test); Different letters on 2 or more bars indicate significant differences between the respective groups (P < 0.05).