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. 2009 Oct 21;106(44):18757–18762. doi: 10.1073/pnas.0910218106

Fig. 4.

Fig. 4.

Tumorigenic potential of wild type cells and MPGES-1 knockdown clones. (A) Clonogenic survival of DU145 and A549 MPGES-1 knockdown clones. Cells were seeded in six-well Cell+ plates and incubated for 12 days in complete growth medium. Colonies (>75 cells) with 50% plate efficiency were counted. Six parallel plates for each cell type and variant were performed, and number of clones ± SE is shown. (B and C) Knockdown of MPGES-1 impairs the tumorigenic potential of DU145 and A549 cells. Kaplan–Meier curves showing the percentage of tumor-free injection sites from the day of tumor cell injection. (B) NMRI nu/nu mice (10 in each group) were injected in both hind flanks with DU145 cells. A significant delay in tumor development was observed in mice xenografted with the MPGES-1 knockdown clone A compared with DU145 WT cells (P < 0.005) or compared with cells transfected with nontargeting shRNA (P < 0.005). (C) NMRI nu/nu mice (10 in each group) were injected in both hind flanks with A549 cells. A significant delay in tumor development was observed in mice xenografted with the MPGES-1 knockdown clone a compared with A549 WT cells (P < 0.005) or compared with cells transfected with nontargeting shRNA (P < 0.005).