Fig. 8.

Proliferation of wild type DU145 cells and MPGES-1 knockdown clone A, effect of adriamycin. (A) Cells were seeded on 96-well plates (10 × 10³ cells per well). At 24 h after seeding, adriamycin (300 nM) was added. After an additional 24 h, cell viability was determined by mitochondrial dehydrogenase assay (cleavage of the tetrazolium salt WST-1; n = 2). (B) PGE₂ augments proliferation of DU145 MPGES-1 knockdown cells in the presence of adriamycin. Cells were seeded on 96-well plates (5 × 10³ cells per well), and 24 h after seeding, adriamycin (300 nM) was added. PGE₂ was added twice, as indicated. After a total 40 h, cell viability was determined by WST-1 assay (n = 2).