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. Author manuscript; available in PMC: 2009 Oct 27.
Published in final edited form as: Development. 2008 Oct 8;135(22):3643–3653. doi: 10.1242/dev.023325

Fig. 9. Robo1 and Robo2 are required for MLF guidance.

Fig. 9

(A-C) MLF labeling adjacent to the floor plate. A single DiI crystal was placed adjacent to the floor plate. (B) In Robo1–/–;Robo2–/– double mutants, DiI labels MLF axons that fan out and cross midline. (C) Close up of wandering axons. (D-F) DiI labeling in the midline of anterior r1 of Robo1–/–;Robo2–/– double mutants back-labels many MLF cell bodies and midline bundles. (The MLF cell bodies are faintly labeled in the example shown.) Note that numerous commissural axons (CA) were labeled in mutant embryos (D, arrowhead), but not in controls. (E) Closeup of back-labeled MLF cell bodies. Their axons project ventrally and bundle in the midline. (F) Closeup of axon bundles in the hindbrain floor plate, and diverging dorsal bundles. Scale bar: 200 μm in A,B,D; 50 μm in C,E,F.