Table 3.
SERIAL CHANGES IN ECHOCARDIOGRAPHIC AND HISTOLOGICAL PHENOTYPES IN β-MyHC-Q403 TRANSGENIC RABBITS
| Non-Transgenic Baseline | Follow up | β-MyHC-Q403 Baseline | Follow up | |
|---|---|---|---|---|
| N | 24 | 24 | 24 | 24 |
| Age (months) | 11.4 ± 4.2 | 24.7 ± 4.2 | 17.9 ± 7.1 | 30.9 ± 7.1 |
| Gender (M/F) | 13/11 | 13/11 | 17/7 | 17/7 |
| Body Weight (Kg) | 3.4 ± 0.3 | 3.8 ± 0.5 | 3.6 ± 0.3 | 3.9 ± 0.4 |
| Heart rate (bpm) | 148 ± 18 | 144 ± 14 | 145 ± 14 | 143 ± 16 |
| IVST (mm) | 2.2 ± 0.3 | 2.2 ± 0.3 | 2.8 ± 0.4 | 3.2 ± 0.5 a,b |
| PWT (mm) | 2.2 ± 0.3 | 2.3 ± 0.3 | 2.89 ± 0.4 | 3.0 ± 0.6b |
| LVEDD (mm) | 14.0 ± 1.8 | 14.5 ± 1.7 | 13.1 ± 2.8 | 15.4 ± 1.8 a |
| LVESD (mm) | 8.6 ± 1.7 | 9.1 ± 1.5 | 8.4 ± 2.3 | 10.9 ± 1.6 a |
| LV Mass (g) | 3.7 ± 0.9 | 3.9 ± 0.6 | 4.4 ± 1.3 | 6.8 ± 2.1 a,b |
| LA Diameter (mm) | 7 ± 0.6 | 7.2 ± 0.6 | 7.4 ± 1.6 | 8.8 ± 2.0 a,b |
| FS (%) | 37.8 ± 7.4 | 37.4 ± 6.3 | 36.0 ± 8.6 | 29.5 ± 3.9 a,b |
| Septal Sa (cm/s) | 8.2 ± 1.7 | 7.1 ± 1.3 c | 5.9 ± 1.6 | 4.8 ± 1.1 a,b |
| Septal Ea (cm/s) | 8.0 ± 1.4 | 7.7 ± 1.4 | 5.3 ± 1.5 | 4.7 ± 1.5b |
| Lateral Sa (cm/s) | 10.9 ± 2.0 | 9.0 ± 1.2 c | 7.6 ± 2.1 | 6.2 ± 1.6 a,b |
| Lateral Ea (cm/s) | 10.1 ± 1.5 | 9.8 ± 1.6 | 6.8 ± 1.5 | 6.5 ± 1.6b |
| Mitral inflow E Vel. (cm/s) | 48.0 ± 6.4 | 50.9 ± 8.9 | 45.7 ± 9.2 | 53.9 ± 7.7 a |
| Mitral inflow E/A ratio | 1.8 ± 0.6 | 1.8 ± 0.5 | 1.7 ± 0.3 | 2.4 ± 0.9 a |
| IVRT (ms) | 40.7 ± 6.6 | 43.3 ± 5.6 | 44.8 ± 7.9 | 39.2 ± 6.1 a |
| Septal E/Ea | 6.2 ± 1.5 | 6.8 ± 1.4 | 9.3 ± 2.8 | 12.8 ± 5.3 a,b |
| Lateral E/Ea | 4.9 ± 1.0 | 5.3 ± 12 | 7.2 ± 0.57 | 8.9 ± 0.57 a,b |
| Histological Phenotypes | ||||
| N | 6-10 | 5-10 | ||
| Myocyte Disarray (%) | NA | 4.4 ± 1.3 | NA | 10.6 ± 3.6d |
| CVF (%) | NA | 3.8 ± 1.7 | NA | 8.2 ± 3.6 |
| Myocyte CSA (μm2) | NA | 366 ± 30 | NA | 475 ± 22d |
The baseline values are as published in Patel et al. Circulation 2001.
a
p <0.05 vs. value obtained in first time point
b
p <0.05 vs. the corresponding value in non-transgenic littermates
c
p <0.01 vs. value obtained in first time point in non-transgenic rabbits
d
p <0.001