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Changes in epithelial cell-cell interactions in cocultures with smokeless tobacco extracts – exposed fibroblasts. HaCAT colonies on control (A, C, and E) or 4% Tob-exposed (B, D, and F) 82.6 dermal fibroblasts were immunostained for E-cadherin (A and B), ZO-1 (C and D), and nuclei (DAPI; E and F). Cocultures of HaCAT and 1%, 2%, or 4% Tob-exposed fibroblasts were also harvested for Western blotting(G).
