Table 2.
Relative numbers of apoptotic nuclei after PDT or treatment with HA14-1
| Treatment | L1210 | L1210/Bax− |
|---|---|---|
| Controls | 2 ± 1 | <1 |
| HA14-1 20 uMa | 9 ± 2 | <1 |
| HA14-1 30 uM | 45 ± 5 | <1 |
| HA14-1 40 uM | 95 ± 3 | 45 ± 3 |
| PDT (LD90)b | 86 ± 4 | 5 ± 1 |
a
Cells were treated with specified levels of HA14-1 for 60 min at 37 °C with HO33342 added during the last 5 min. Numbers of apoptotic nuclei were determined by counting three fields of 100 cells each.
b
Cells were given an LD90 PDT dose using CPO, incubated for an additional 60 min with numbers of apoptotic nuclei determined as described above.