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. 2009 Oct 5;10:63. doi: 10.1186/1471-2156-10-63

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Copyright © 2009 Zhou et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Erythrocyte membrane protein band 4.1-like 3 (EPB41L3) splicing alteration. EPB41L3 was selected from a set of genes that displayed a large difference at the gene level between samples A and B, and, in addition, had event ratios that were significantly higher than 3 fold between these samples, indicating a splicing alteration. (A) Using the UCSC browser visualization feature of the SpliceArray™ portal (http://portal.splicearray.com), the event is illustrated with the variant sequence used to identify the event (red), the reference sequence (black), the probe target regions (blue), and the PCR primers (arrowheads) used to validate the event. (B) RT-PCR results for the event validation using the primers indicated in A. R = reference and V = variant (C) Protein domain analysis was done using CDART [38] and shows a portion of the C terminal region is removed and may affect the function of the protein. (Note that Event ID 23136.004 = Entrez Gene ID.ID1 and probe sets are additionally labeled with F, T, B, C, D, or E.)