Figure 1. TGF-β stimulates Nab2 expression.

Confluent cultures of human foreskin fibroblasts were incubated with TGF-β1 (10 ng/ml) for indicated periods. A, D Total RNA was isolated and analysed by real-time quantitative PCR. Results, normalized with actin, are the means±S.D. of triplicate determinations from a representative experiment. B. Whole cell lysates were subjected to Western analysis. Representative immunoblots. C. Fibroblasts were fixed and stained with anti-Nab2 antibodies, or DAPI to detect nuclei, and viewed by immunofluorescence microscopy (original magnification ×100).