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. 2007 May 23;13:758–763.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ethidium bromide-stained agarose gel of ciliary neurotrophic factor and the ciliary neurotrophic factor tripartite receptor complex polymerase chain reaction products from optic nerve head cells. Lamina cribrosa cells are lanes 1-3, optic nerve head astrocytes (ONHA) are lanes 4-7, brain astrocytes are lane 8, and optic nerve head tissue (ONA tissue) is lane 9. C indicates negative control (i.e. no template reaction control). L indicates molecular size ladder. Each lane corresponds to an individual donor. Lamina cribrosa (LC) cells are from donors of 8 month old (8 mth LC), 66 year old (66 yr LC), and 90 year old (90 yr LC). ONHA cells are from donors of 36 week old (36 wk ONA), 66 year old (66 yr ONA), 89 year old (89 yr ONA), 90 year old (90 yr ONA). β-actin (350 bp) was included to monitor potential genomic DNA contamination.