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. 2007 May 23;13:764–771.

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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Topological correspondence of Ca_v1.3 (α1D) in situ hybridization probes. Voltage-dependent calcium channel pore-forming α1 subunits are composed of a single polypeptide consisting of 4 repeating domains (I-IV) joined by intracellular loops. Each repeat domain consists of 6 transmembrane-spanning regions (1-6) connected by intracellular and extracellular loops. Antisense and sense probe sets were generated using cRNA corresponding to 2 different topological regions of the Ca_v1.3 (α1D) subunit polypeptide. Probe A corresponded to cRNA encoding an N terminal fragment of the polypeptide, while B corresponded to cRNA encoding a fragment of the variable loop between repeat domains II and III. Probe B encompassed the antigenic peptide (*) used to generate the commercially available antibody used in most published immunohistochemical studies of Ca_v1.3 (α1D) expression.