TABLE I.

Rates for current simulations using 10-state activation model

4 µM Ca2+	C1–C2	C2–C3	C3–C4	C4–C5	zJ	zL
α(0)c	4,480	3,360	2,240	1,120	0.29 e
β(0)c	30,000	60,000	90,000	120,000	−0.29 e
	C1–O1	C2–O2	C3–O3	C4–O4	C5–O5
δ(0)	0.4	7.344	120	288	350	0.212 e
γ(0)	3,200	2,350.08	1,536	147.456	7.168	−0.088 e
	O1–O2	O2–O3	O3–O4	O4–O5	z
α(0)o	22,400	16,800	11,200	5,600	0.29 e
β(0)o	6,000	12,000	18,000	24,000	−0.29 e
300 µM Ca2+	C1–C2	C2–C3	C3–C4	C4–C5	zJ
α(0)c	6,400	4,800	3,200	1,600	0.29 e
β(0)c	12,000	24,000	36,000	48,000	−0.29 e
	C1–O1	C2–O2	C3–O3	C4–O4	C5–O5
δ(0)	24	340	6,000	6,000	6,000	0.212 e
γ(0)	2,227	841.31111	395.9111	10.5576	0.2815368	−0.088 e
	O1–O2	O2–O3	O3–O4	O4–O5	z
α(0)o	40,000	30,000	20,000	10,000	0.29 e
β(0)o	2,000	4,000	6,000	8,000	−0.29 e

Numbers are rates (s⁻¹) for the indicated transitions (e.g., C₁–C₂, state C1 to state C2). Calculated allosteric constants based on microscopic rates: L′ (4 µM) = 0.000125; D′ (4 µM) = 25; J′ (4 µM) = 0.037333; L′ (300 µM) = 0.0107768; D′ (300 µM) = 37.5; J′ (300 µM) = 0.1333333; z_L = 0.3 e; z_J = 0.58 e.