TABLE III.

Model-dependent parameters resulting from fit of GV curves

Basic open-channel block
Scheme 1	Scheme 1a
Kbo (µM)	5.90 ± 0.40
zc	0.13 ± 0.02 e
Kbc (µM)
zc
SSQ	395.95
State-independent closed- and open-channel block and variations
Scheme 2	Scheme 2a	Scheme 2a′	Scheme 2b	Scheme 2b′
Kbo (µM)	7.42 ± 0.43	6.01 ± 0.30	6.96 ± 0.39	6.36 ± 0.45
zc	0.17 ± 0.02 e	0.10 ± 0.02 e	0.15 ± 0.2 e	0.15 ± 0.02 e
Kbc (µM)	=Kbo	45.79 ± 35.9	=Kbo	21.4 ± 12.2
zc	=zc	0.81 ± 0.20 e	0.0*	0.0*
SSQ	309.199	100.66	486.663	326.09
Block dependent on movement of one voltage sensor
Scheme 3	Scheme 3a	Scheme 3a′	Scheme 3b	Scheme 3b′
Kbo (µM)	6.14 ± 0.26	6.01 ± 0.27	6.02 ± 0.29	6.48 ± 0.29
zo	0.13 ± 0.01 e	0.10 ± 0.02 e	0.13 ± 0.02 e	0.13 ± 0.01 e
Kbc (µM)	=Kbo	8.1 ± 6.7	=Kbo	2.34 ± 0.38
zc	=zc	0.44 ± 0.23 e	0.0	0.0*
SSQ	159.163	93.75	209.18	138.242
Block scales with movement of each voltage sensor
Scheme 4		Scheme 4a′		Scheme 4b′
4 Kbo (µM)		19.53 ± 0.9		20.78 ± 0.80
zo		0.06 ± 0.02 e		0.08 ± 0.01 e
4 Kbc (µM)		7.4 ± 5.2		3.05 ± 0.44
zc		0.30 ± 0.21 e		0.0*
SSQ		90.3005		106.822
Closed-channel block scales with movement of each voltage sensor; open channels are blocked identically
Scheme 5		Scheme 5a′		Scheme 5b′
Kbo (µM)		6.03 ± 0.28		6.38 ± 0.26
zo		0.10 ± 0.02 e		0.12 ± 0.01 e
4 Kbc (µM)		8.52 ± 6.4		3.21 ± 0.48
zc		0.33 ± 0.23 e		0.0*
SSQ		95.99		121.019
Closed channel block depends on movement of two voltage sensors
Scheme 6		Scheme 6a′		Scheme 6b′
Kbo (µM)		6.04 ± 0.24		5.70 ± 0.23
zo		0.10 ± 0.02 e		0.069 ± 0.01 e
4 Kbc (µM)		0.02 ± 0.02		.174 ± 0.02
zc		0.62 ± 0.23 e		0.0*
SSQ		89.91		124.9026