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. 2009 Oct 26;206(11):2455–2467. doi: 10.1084/jem.20090805

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© 2009 Kunder et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — MC-derived particles contain preformed TNF. (A) Differential interference contrast micrograph of purified MC granule particles. (B) SYPRO Ruby–stained SDS-PAGE gel of MC particle-associated proteins. (C) TNF immunoblot of SDS-PAGE–separated proteins from purified particles showing detection of rat TNF. (D) A still image from Video 2 showing that particles released from RBL-2H3 cells after activation retain overexpressed TNF-GFP fusion protein. Bars, 15 µm. White boxes highlight released particles with retained fluorescence. All panels depict data representative of experiments performed more than three times, with trials using lavage from individual rats or pooled from multiple rats, for a total n > 3.