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. 2009 Oct 26;206(11):2469–2481. doi: 10.1084/jem.20090525

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© 2009 Jenne et al.

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Figure 2. — Peripheral lymph node NK cell accumulation in Duane results from a cell-intrinsic egress defect. (A) Analysis of wild-type mice reconstituted with a mix of wild-type and Duane BM. Some mice (PLN+Block) received integrin and selectin blocking antibodies 40 h before analysis. Values are reported as a ratio of Duane (CD45.2) to wild-type (CD45.1) cells. At least two untreated and two treated chimeras were analyzed in each of two independent experiments. (B) Analysis of wild-type B6 mice 24 h after adoptive transfer of either Duane or wild-type splenocytes. Values are reported as a ratio of transferred NK cells recovered from the spleen (as a percentage of transferred B cells) to transferred NK cells in the peripheral LNs (as a percentage of transferred B cells). Data were obtained from two independent experiments, each composed of two recipients receiving Duane splenocytes and two recipients receiving wild-type splenocytes. Bars represent mean values, and circles represent individual animals. PLN, peripheral lymph node. *, P < 0.005; **, P < 0.001.