Figure 2.

Effect of Ir-CPI on the thrombin activity profile during the coagulation process induced either by intrinsic or extrinsic coagulation pathway. (A) Human plasma was incubated with various concentrations of Ir-CPI (0, 0.001, 0.003, 0.009, 0.027, 0.081, 0.243, 0.729, 2.187, 6.561, and 9.077 µM), and the mixture was activated with ellagic acid and PL to initiate the intrinsic coagulation pathway. (B) Human plasma was incubated with various concentrations of Ir-CPI (0, 0.729, 2.187, 6.561, and 9.077 µM), and the mixture was activated with 5 pM TF and PL to initiate the extrinsic coagulation pathway. The amidolytic activity of thrombin was determined by adding its specific fluorogenic substrate and recording the increase in fluorescent signal. Results are presented as the mean of three independent experiments.