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. 2009 Oct 26;206(11):2483–2496. doi: 10.1084/jem.20091046

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© 2009 Morikawa et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 2. — Identification of MSC potential by clonal assay. (A) Phase-contrast micrographs of a representative colony from a single PαS cell. Bar, 200 µm. (B) Comparison of the differentiation potential of clonally derived cells. Adipogenic (day 14), chondrogenic (day 21), osteogenic (day 14), and endothelial (anti-PECAM-1⁺ and VE-cadherin⁺, day 21). Bars, 100 µm. (C) RT-PCR analysis of transcription factors and lineage-specific genes. Expression of adipocyte- (Adipsin, PPARγ, and mLP), chondrocyte- (CollagenII, CollagenX, and Aggrecan), and osteocyte-specific (Osteopontin, Osteocalcin, and PTHr) markers, 3 wk after differentiation induction. Data are representative of five independent experiments.