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. 2009 Oct 26;206(11):2483–2496. doi: 10.1084/jem.20091046

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© 2009 Morikawa et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jem.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — In vivo effects of lethal irradiation on the quiescence of PαS cells. HSCs (CD34⁻KSL) from B6-Ly5.1 (CD45.1) mice and PαS cells (MSCs) from CAG-EGFP transgenic mice were transplanted together into lethally irradiated B6-Ly5.2 mice to examine the competitive repopulation of the appropriate niches. The graph shows the percentage of CD45.1 and GFP donor-derived cells detected in the BM of recipient mice 16 wk after transplantation (CD45.1, 81.1 ± 4.95%; GFP⁺, 7.4 ± 0.40%.; n = 3 per group in three independent experiments). (B) The numbers of HSCs (KSL, c-Kit⁺Sca-1⁺Lin⁻) and MSCs (PαS, PDGFRα⁺Sca-1⁺) in the BM were calculated by ([Total number of BMMNCs] × [% of the cells] /100). Black bar, untreated control mice; gray bar, irradiated mice, 10 d after lethal irradiation. Results are means ± SEM (n = 3 per group). (C) The number of CFU-Fs formed from PαS cells isolated from either of unirradiated control (black) or lethally irradiated (Gray) wild-type C57BL/6. Means ± SEM (n = 3 per group). (D) Representative flow cytometric analysis of HSCs (KSL, top) or MSCs (PαS, bottom) in BMMNCs of lethally irradiated or unirradiated control mice. (E) Flow cytometry analysis of PY/Hoechst-stained PαS cells of unirradiated (left) and lethally irradiated (right) mice. Data are representative of three independent experiments.