Table 3. Assessment of dCt cut-off values for mutation calling with the KRAS-TMGB test in FFPE-DNA samples. Mutant FFPE-DNA samples validated with sequencing.

		dCt mut
assay	n samples	mean	SD	calculated cut-offˆ
12ALA	5	−0,7132	0,6340	1,1887
12ARG	3	−0,9212	0,4115	0,3132
12ASP	9	0,0646	1,0771	3,2960
12CYS	2	−0,0295	0,9033	2,6804
12SER	3	1,2942	1,2642	5,0868
12VAL	16	0,9986	1,3690	5,1057
13ASP	6	−0,2423	0,8264	2,2368

ˆ  =  mean + 3X SD.