Table 4. Detailed assessment of dCt cut-off values for mutation calling with the KRAS-TMGB test. Mutant FFPE-DNA samples^* were diluted 1∶10 and 1∶100 in wild-type samples. Correspondingly, final concentrations of 7% and 0.7% tumor cells were anticipated.

	dCt, undiluted		dCt, 1∶10				dCt, 1∶100
assay	mean	SD	mean	SD	calculated cut-offˆ	cut-off for use	mean	SD
12ALA	−0,0992	0,2284	0,3386	0,2203	0,9997	<2	1,2900	0,1531
12ARG	−1,9221	0,0211	−0,1348	0,1863	0,4240	<2	1,4814	0,1318
12ASP	−0,3009	0,1802	3,4264	0,1093	3,7543	4	7,6930	0,3609
12CYS	0,7338	0,1737	3,5420	0,0616	3,7269	4	5,4607	0,1741
12SER	−0,1940	0,1407	4,3250	0,1537	4,7862	5	6,3535	0,4833
12VAL	0,4837	0,1256	4,3814	0,1881	4,9458	5	6,6008	0,9939
13ASP	−0,6969	0,0817	2,4194	0,1543	2,8823	3	4,2344	0,3250

* =  mutant samples, sequencing validated, containing approximately 70% tumor cells.

ˆ  =  mean + 3X SD.