Figure 4.

LxS-MYB305 Is a Transcriptional Activator in Yeast and It Activates the Transcription of Reporter Genes via Its C-Terminal Domain.

(A) Constructs used in these studies. The GAL4 DNA binding domain (DB) and LxS-MYB305-Δ fragment (MYB-Δ) fusion genes driven by the ADH1 promoter serve as effectors; the HIS3 and LacZ (data not shown) genes with Gal4 DNA binding site on their promoters serve as reporters. The constructs of each effector are shown with DNA fragments identified above the individual constructs. R2 and R3 represent the R2 and R3 repeat of MYB DNA binding domains, respectively; AD represents the activation domain. The + control contains a recombined Gal4 DNA binding domain and activation domain; the – control contains Gal4 DNA binding domain only.

(B) Serial dilution growth assays of transformed yeast on selective YSM His⁻ + 10 mM 3AT medium. Constructs are identified to the left of the figure. Dilutions are identified below the figure.

(C) Quantitation of β-galactosidase activity in yeast. Constructs are identified below the bars (means ± sd, n = 4 with two replicates for each sample). +, positive control construct; −, negative control construct.

[See online article for color version of this figure.]