Temporal Expression of myb305 in Nectaries of Wild-Type LxS8 Plants.
(A) RT-PCR of myb305, nec1, and nec5 in the nectary glands of LxS8 plants at different floral stages. Both 18S and 26S rRNAs were used as controls. PF = 48 h postfertilization. Amplification cycle number for 18S rRNA, 26S rRNA, and myb305 is 24 for all floral stages. Amplification cycle number for nec1 and nec5 is 26 for all floral stages.
(B) Protein blotting of MYB305 and NEC1 proteins in the nectary glands of LxS8 plants at different floral stages. Film exposure for MYB305 was 20 min. Film exposure for NEC1 was 30 min.