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. 2009 Sep;21(9):2672–2687. doi: 10.1105/tpc.108.060079

Figure 9.

Figure 9.

Analysis of LxS-MYB305 Overexpression and RNAi Plants.

(A) Quantitative RT-PCR analysis of LxS-myb305, nec1, nec5, pal, and chi expression in the foliage of LxS-MYB305 overexpression N. tabacum plants. Means + sd are shown (four biological replicates with two technical replicates each).

(B) Quantitative RT-PCR analysis of LxS-myb305, nec1, nec5, pal, and chi expression in the stage 12 nectary glands of LxS-MYB305 RNAi N. tabacum plants. Means + sd are shown (four biological replicates with two technical replicates each).

(C) Immunoblotting of MYB305 and NEC1 proteins in the foliage of LxS-MYB305 overexpression N. tabacum plants. Film exposure time was 1 h for MYB305 and 12 h for NEC1.

(D) Immunoblotting of MYB305 and NEC1 proteins in the stage 12 nectary glands of LxS-MYB305 RNAi N. tabacum plants. Film exposure for both MYB305 and NEC1 was 30 min.

(E) Phenotype comparison of wild-type N. tabacum nectary and RNAi N. tabacum nectary. Bar = 2 mm.