Cryptochromes Are Required for Blue Light–Triggered Stomatal Development.
(A) Differential interference contrast (DIC) images of the abaxial cotyledon epidermis of 10-d-old wild-type, cry1 cry2 double mutant, and 35Spro–CRY1 seedlings. Seedlings were grown in blue light (30 μmol·m−2·s−1), red light (50 μmol·m−2·s−1), and far-red light (12 μmol·m−2·s−1). Meristemoids are indicated by arrowheads. Bars = 20 μm.
(B) The SI obtained from the samples in (A). In (B) to (D), the SI and SMI are presented as the percentage of mean ± sd. Asterisks denote significant differences between the indicated genotypes and the wild type (t test, P < 0.01), n = 10.
(C) The SMI obtained from the samples in (A). Asterisks denote significant difference between cry1 cry2 and the wild type (t test, P < 0.01), n = 10.
(D) The SI of the abaxial leaf epidermis of ∼4-week-old wild-type, 35Spro–CRY1, and cry1 cry2 plants grown under blue light (50 μmol·m−2·s−1). Asterisks denote significant differences between cry1 cry2 and the wild type (t test, P < 0.01), n = 5.