Figure 2.

Arabidopsis Plants with Reduced POLδ1 Expression Show Increased HRFs.

Arabidopsis lines with epigenetic POLδ1 alleles were generated by the integration of three different RNAi-mediating vector constructs (top panel) into a GUS-based HR reporter line with inverted repeats (line IR1). P_mas, mannopine synthase; P_alc, recombinant alcohol-inducible promoter; P_U6, Arabidopsis U6 snRNP promoter. Two weeks after germination, the HRF and the POLδ1 expression level were assessed.

(A) The steady state levels of POLδ1 transcripts were analyzed by quantitative RT-PCR. The expression data of mutant lines was plotted relative to their respective vector controls (C lines). Note: induction of the P_alc promoter was not required to reduce POLδ1 expression.

(B) Recombination events per plant were assessed in independent plant lines and are shown relative to their respective vector controls (C lines). Error bars indicate standard error of independent experiments (n ≥ 3). Asterisks indicate statistically significant differences compared with controls.