Figure 5.

Weak polδ1 Alleles Enhance HRF in a Substrate-Specific Manner.

(A) Scheme of GUS-based HR substrates differing in the arrangement of the repeats with respect to each other. Intra- and intermolecular interactions between IRs and DRs, respectively, give rise to the restoration of a functional marker gene through HR. By contrast, TRs allow repair of breaks by both intra- and intermolecular HR as well as by single-strand annealing.

(B) Plants with either WHR (left and central panels) or SHR (right panel) polδ1 alleles were crossed with substrate lines. HR events were assessed in 2-week-old F1 offspring, which were hemizygous for both the mutation and the substrate locus. Error bars indicate standard error of HR events/plant (n ≥ 6 plants). Asterisks indicate significant differences between mutant and wild-type control plant population by ANOVA.