Table 3.

Expression Analyses of polδ1 Mutant Plants: Expression of DNA Repair and Checkpoint Factors by Quantitative RT-PCR Analysis

		Relative Expression (±se) in polδ1 Mutant Plants
Gene		WHR Class Mutants		SHR Class Mutants
Name	ID	polδ1-1 (+/−)	RNAi-POLδ1-1	RNAi-POLδ1-3	RNAi-POLδ1-4	RNAi-POLδ1-5
RAD51	At5g20850	1.20 (±0.065)	1.12 (±0.017)	1.16 (±0.043)	1.00 (±0.037)	1.00 (±0.098)
BRCA1	At4g21070	0.83 (±0.097)	1.18 (±0.149)	1.70 (±0.196)	1.40 (±0.077)*	1.17 (±0.169)
PARP2	At4g02390	1.18 (±0.273)	2.00 (±0.411)	1.74 (±0.208)*	1.66 (±0.181)*	1.84 (±0.45)
ATR	At5g40820	1.05 (±0.439)	1.34 (±0.512)	1.84 (±0.117)	1.88 (±0.157)	0.73 (±0.095)
RAD17	At5g66130	1.24 (±0.045)	0.83 (±0.076)	0.83 (±0.013)	1.36 (±0.119)	1.14 (±0.128)
RNR2A	At3g23580	2.19 (±0.231)	1.25 (±0.079)	1.60 (±0.103)*	1.25 (±0.388)	0.65 (±0.061)

For polδ1 mutant plants, the steady state transcription levels of selected DNA transaction-related genes were analyzed by quantitative RT-PCR. Asterisks indicate statistically significant changes comparing experimental replica (n ≥ 3) of wild-type and mutant plants by the Student's t test.