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. 2009 Sep;21(9):2859–2877. doi: 10.1105/tpc.109.066118

Figure 2.

Figure 2.

Selective BiFC of KC1 with SYP121 Expressed in Arabidopsis Root Epidermis as Fusion Constructs with the N- and C-Terminal Halves of YFP (nYFP and cYFP), Respectively.

(A) Three-dimensional reconstructions from confocal fluorescence image stacks of seedlings expressing (bright-field, left; fluorescence, right) SYP121-nYFP with KC1-cYFP ([a] and [b]), SYP122-nYFP with KC1-cYFP ([c] and [d]), and with the empty (untransformed) Agrobacterium ([e] and [f]). Bar = 50 μm. Images with SYP111-nYFP paired with KC1-cYFP were similar to those of (c) and (d).

(B) Mean fluorescence intensities (arbitrary units) ± se after correction for background of (nontransfected) control measurements (n > 6 independent experiments in each case; * indicates significant difference from the empty control at P < 0.01). See also Supplemental Figure 2 and Supplemental Movie 1 online.