Figure 2.
Screening statistics and candidate selection. (a) Screening statistics. The table lists the number of successful replicates per clone. (b) Selection of clones with non-random luminescence variation. All luminescence ratios were transformed into log-space for visualization. Luminescence ratios with a negative log value indicate a repressive effect, and positive log values an activating effect. The dotted line represents a Gaussian normal distribution fitted to the dataset. The left vertical line labels the threshold for repressors (less than 10-0.544 = 0.2859), and the right vertical line labels the threshold for activators (more than 100.458 = 2.8732) (c) Gene Ontology analysis of candidate regulators. Candidates were analyzed for cellular localization and molecular function independently. The most abundant, non-redundant categories with a significant enrichment in the dataset compared to the genome are depicted.