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PM-IRRAS analysis of each step of the localized SAM replacement process. (A) 100% HS—EG3—OH terminated SAM, (B) following removal of SAM via aqueous NaBH4, (C) bare gold for comparison, (D) following formation of a new SAM using an aqueous 25 mol % HS—EG6—COOH alkanethiolate solution, and (E) following conjugation of RGDSP via carbodiimide condensation.
