Fig. 1.

Expression of the different mouse sPLA₂s in the intestine of C57BL/6J and BALB/c mice by RT-qPCR and in situ hybridization of mGX sPLA₂. A and B, RT-qPCR in C57BL/6J (A) and BALB/c (B) mouse intestine sections using specific sets of PLA₂ primers. To facilitate the comparison of expression between the different sPLA₂s, the data were first normalized to glyceraldehyde-3-phosphate dehydrogenase mRNA, which was used as a reference gene, and then expressed relative to the lowest expression level that can be accurately measured in our RT-qPCR assay conditions [i.e., the expression of pancreatic group IB sPLA₂ in the colon {relative abundance of 1 (arbitrary unit = 1)}]. Note that two different ordinate axes have been used in B. mGIIE sPLA₂ could not be detected in all intestine sections from BALB/c mice (C–F). In situ hybridization of mGX sPLA₂ in small intestine (ileum) showing labeling of columnar epithelial cells in mucosal villi (V), Paneth cells (P), and ganglion cells (G) of the myenteric plexus. Hybridized with antisense probe. D, absence of reaction product from ileal tissue when hybridized with sense probe. E, in situ hybridization of mGX sPLA₂ in large intestine (cecum) showing labeling in epithelial cells. Hybridized with antisense probe.