Fig. 2.

Effect of group X sPLA₂ on [³H]thymidine incorporation in mouse colon cell lines. After starvation for 24 h at 37°C in serum-free and additive-free medium, all cell lines were incubated in the presence of mGX and hGX sPLA₂s (200 nM) or FCS under the following conditions: Colon-26 were grown at 37°C for 24 h in DMEM and 0.02% FAF BSA (A); AJ02-nm0 cells were grown at 37°C for 48 h in RPMI 1640 medium and 0.02% FAF BSA (B); Apc(+/Min) cells were grown at 33°C in DMEM, 0.02% FAF BSA, and 10 ng/ml recombinant EGF (C); and YAMC cells were incubated at 33°C for 48 h in serum-free RPMI 1640 medium and 0.02% FAF BSA (D). [³H]Thymidine was added during the last 4 h of sPLA₂ stimulation, and cells were processed as described under Materials and Methods. The increase in [³H]thymidine incorporation induced by sPLA₂s was compared with untreated cells (−) and FCS-treated cells. In all panels, values are representative of at least two experiments performed in triplicates. Significant differences between untreated and sPLA₂-treated cells were found (**, P < 0.001; Student's t test).