Fig. 6.

Release of AA by various mouse sPLA₂s and production of eicosanoids triggered by mGX sPLA₂ from Colon-26 cells. A, release of AA by various mouse sPLA₂s was measured on quiescent Colon-26 cells prelabeled with [³H]AA for 24 h as described under Materials and Methods. Mouse recombinant sPLA₂s (200 nM) were incubated with cells for 6 h in DMEM with 0.02% FAF BSA. [³H]AA release is expressed as the percentage of radioactivity present in cell medium relative to the total radioactivity incorporated into cells. sPLA₂-X-treated cells release significantly more [³H]AA (**, P < 0.001; one-way ANOVA, with Bonferroni adjustment). A representative experiment of at least two experiments is shown. B, eicosanoid production triggered by mGX sPLA₂. Colon-26 cells (10⁶ cells) were starved for 24 h and treated with mGX sPLA₂ (200 nM) for 24 h in DMEM and 0.02% BSA. The cell medium and the cell monolayer were collected, extracted with organic solvent, combined, and analyzed for eicosanoid production by liquid chromatography/mass spectrometry as described under Materials and Methods. A representative experiment of two experiments is shown.