Fig. 7.

Release of lysophospholipids by group X sPLA₂ from Colon-26 cells. A, total amount of LPX acyl chain species released by hGX sPLA₂. B, release of the different LPC and LPA acyl chain species by hGX sPLA₂. Colon-26 cells were starved for 24 h and treated with hGX sPLA₂ (200 nM) for 6 h in DMEM and 0.02% BSA. The cell medium and the cell monolayer were collected, extracted with organic solvent, combined, and analyzed for lysophospholipid production by liquid chromatography/mass spectrometry as described under Materials and Methods. A representative experiment of two experiments is shown.