Fig. 6.
[125I]Ghrelin binds to and dissociates from the ghrelin receptor in a monophasic fashion. A, the specific binding at 4°C of 125I-ghrelin to membranes of HEK293 cells coexpressing Gαo1, and the ghrelin receptor was measured over time. Data were fitted to a monophasic hyperbola consistent with Kobs = 0.029 min−1. Data points represent mean ± S.E.M of three independent experiments performed in triplicate. B, after association of 125I-ghrelin as above for 120 min, dissociation of the ligand was measured over time after addition of 10−6 M ghrelin. Data are presented as a semi-log plot. Koff = 0.02 ± 0.002 min−1. Data points represent the mean of three independent experiments performed in triplicate.